| 弓形虫新基因wx2表位疫苗免疫小鼠的保护研究 |
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| 引用本文： | 谭逵,张 琼,范久波,谢荣华,蒋立平,吴 翔,舒衡平.弓形虫新基因wx2表位疫苗免疫小鼠的保护研究[J].生物化学与生物物理进展,2008,35(9):1051-1058. |
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| 作者姓名： | 谭逵 张 琼 范久波 谢荣华 蒋立平 吴 翔 舒衡平 |
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| 作者单位： | 中南大学湘雅医学院寄生虫学教研室,长,沙,410078 |
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| 基金项目： | 国家自然科学基金，湖南省重大专项基金，湖南省重点学科建设经费联合资助项目 |
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| 摘 要： | 采用牛物信息学方法对弓形虫新基因wx2进行表位分析预测,PCR扩增基因中编码2个表位的片段w2b和w2a,成功构建新基因的单表位疫苗质粒pcDNA3-W2b、pcDNA3-W2a和双表位疫苗pcDNA3-w2b2a,接种小鼠,观察表位疫苗的免疫保护作用.将表位疫苗分别通过肌肉注射免疫小鼠,对照组注射pcDNA3空质粒.ELJSA法检测血清IgG抗体水平,取脾细胞用流式细胞仪检测T淋巴细胞亚群.各组小鼠末次免疫后第4周每只小鼠经腹腔注射弓形虫速殖子500个,观察小鼠的生存时间.结果显示,pcDNA3-W2a2b双表位疫苗组小鼠血清IgG抗体水平显著高于对照组(P＜0.05),且pcDNA3-W2a2b双表位疫苗组小鼠脾细胞CD4 T与CD8 T淋巴细胞比值明显低于两单表位疫苗组,pcDNA3-W2a2b双表位疫苗组小鼠存活时间明显长于两单表位疫苗组(P＜0.05).实验结果表明,弓形虫新基因wz2表位疫苗能够诱导小鼠产生抗弓形虫感染保护性免疫,并且弓形虫poDNA3-W2b2a双表位疫苗的免疫保护性优于pcDNA3-W2b、pcDNA3-W2a两单表位疫苗.
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| 关 键 词： | 弓形虫 表位疫苗 重组质粒 B细胞表位 保护力 |
| 收稿时间： | 2008/1/11 0:00:00 |
| 修稿时间： | 2008/4/22 0:00:00 |
The Protective Efficacy Induced by The Epitope Vaccines From New Gene wx2 of Toxoplasma gondii in Mice |
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| tankui,ZHANG Qiong,FAN Jiu-Bo,XIE Rong-Hu,JIANG Li-Ping,WU Xiang and SHU Heng-Ping.The Protective Efficacy Induced by The Epitope Vaccines From New Gene wx2 of Toxoplasma gondii in Mice[J].Progress In Biochemistry and Biophysics,2008,35(9):1051-1058. |
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| Authors: | tankui ZHANG Qiong FAN Jiu-Bo XIE Rong-Hu JIANG Li-Ping WU Xiang and SHU Heng-Ping |
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| Institution: | Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China;Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China;Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China;Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China;Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China;Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China;Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China |
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| Abstract: | |
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| Keywords: | Toxoplasma gondii epitope vaccine recombinant plasmid B lymphocyte epitope protective efficacy |
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