The expression of cecropin peptide in transgenic tobacco does not confer resistance to Pseudomonas syringae pv tabaci |
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Authors: | Robin Hightower Catherine Baden Eva Penzes Pamela Dunsmuir |
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Institution: | (1) DNA Plant Technology Corporation, 6701 San Pablo Avenue, 94608 Oakland, California, USA;(2) Present address: Department of Plant Pathology, North Carolina State University, Raleigh, N.C., USA |
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Abstract: | Summary We used in vitro growth inhibition assays to demonstrate that synthetic cecropin protein has potent activity against a range of plant pathogenic bacteria. We then prepared transgenic tobacco plants which express cecropin mRNA and protein. We have used Pseudomonas syringae pv tabaci infection of these transgenic tobacco as a model system to evaluate whether the plants which express cecropin protein also have increased tolerance to infection. We found no dramatic difference in disease response between plants which are expressing cecropin protein and control plants which were derived from the transformation with a binary vector which did not carry the gene encoding cecropin protein. |
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Keywords: | Cecropin Transgenic tobacco Pseudomonasas syringe pv tabaci |
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