Culturable actinomycetes from desert ecosystem in northeast of Qinghai-Tibet Plateau |
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Authors: | Dong Ding Guichen Chen Bochan Wang Qilan Wang Demei Liu Min Peng Ping Shi |
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Institution: | 1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, People’s Republic of China 2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China
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Abstract: | In this study, 53 actinomycetes strains were isolated from desert ecosystems located in northeast of Qinghai-Tibet Plateau and grouped into four RFLP patterns. Twenty-six actinomycetes with obvious morphology differences were chosen for phylogenetic diversity study and antimicrobial testing. As a result, the 16S rRNA gene sequencing showed that these strains belonged to Streptomyces, Micromonospora, Saccharothrix, Streptosporangium and Cellulomonas, and that most of the strains had antimicrobial bioactivity. The PKS and NRPS genes detection indicated diversified potential bioactive products of actinomycetes in this ecosystem. Among these strains, Sd-31 was chosen to study the bioactive products using HPLC-MS because of its optimum antimicrobial bioactivity. The result showed that it might produce Granatomycin A, Granatomycin C, and an unknown compound. |
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