| 内生真菌对姜黄素的微生物转化 |
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| 引用本文： | 彭清忠,陈玲,易浪波,陈义光,彭清静.内生真菌对姜黄素的微生物转化[J].生物技术通讯,2010,21(2):196-199. |
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| 作者姓名： | 彭清忠 陈玲 易浪波 陈义光 彭清静 |
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| 作者单位： | 吉首大学,生物资源与环境科学学院,植物资源保护与利用湖南省高校重点实验室,湖南,吉首,416000 |
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| 基金项目： | 国家自然科学基金，湖南省重点学科建设项目 |
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| 摘 要： | 目的:调查姜黄根茎内生真菌对姜黄素的微生物转化,以期获得一些姜黄素的结构类似物或衍生物。方法:利用表面消毒法分离内生真菌;采用薄层层析和高效液相色谱(HPLC)技术筛选生物转化姜黄素的内生真菌;利用硅胶柱层析和制备型HPLC分离纯化生物转化产物;应用波谱技术解析转化产物的化学结构;通过形态学特征和内转录间隔区(ITS)序列分析对内生真菌进行初步鉴定。结果:从姜黄根茎中分离出18株内生真菌,经筛选发现其中1株丝状真菌能转化姜黄素,其产物分别为去甲基姜黄素和二去甲基姜黄素。初步鉴定该内生真菌属于Diaporthe sp.。结论:内生真菌Diaporthe sp.能对姜黄素进行去甲基化修饰,推测它可能具有O-去甲基化酶系。
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| 关 键 词： | 姜黄素 微生物转化 内生真菌 Diaporthe sp． |
Transformation of Curcumin by Endophytic Fungus Diaporthe sp.Isolated from Curcuma Longa |
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| PENG Qing-Zhong,CHEN Ling,YI Lang-Bo,CHEN Yi-Guang,PENG Qing-Jing.Transformation of Curcumin by Endophytic Fungus Diaporthe sp.Isolated from Curcuma Longa[J].Letters in Biotechnology,2010,21(2):196-199. |
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| Authors: | PENG Qing-Zhong CHEN Ling YI Lang-Bo CHEN Yi-Guang PENG Qing-Jing |
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| Institution: | PENG Qing-Zhong,CHEN Ling,YI Lang-Bo,CHEN Yi-Guang,PENG Qing-Jing College of Biology , Environmental Sciences,Jishou University,Hunan Provincial Key Laboratory of Plant Resources Conservation , Utilization,Jishou 416000,China |
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| Abstract: | Objective:The microbial transformation of curcumin by endophytic fungi isolated from the rhizoma of Curcuma longa was investigated to obtain some curcuminoids or its derivatives.Methods:Endophytic fungi were isolated from the rhizoma of C.longa by surface-sterilization method,and screening test for the microbial transformation of curcumin was carried out using thin layer chromatography and high performance liquid chromatography (HPLC).Bio-transformed products of curcumin were isolated and purified by silica... |
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| Keywords: | Diaporthe sp |
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