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Advances in soil microbial ecology and the biodiversity
Authors:Tsutomu Hattori  Hisayuki Mitsui  Hideki Haga  Norio Wakao  Shuichi Shikano  Krystyna Gorlach  Yasuhiro Kasahara  Adel El-Beltagy  Reiko Hattori
Affiliation:(1) Institute of Genetic Ecology, Tohoku University, Katahita 2-1-1, Aoba-Ku, Sendai, 980, Japan;(2) Agricultural Faculty, Iwate University, Ueda 4-16-43, Morioka, 020, Japan;(3) Department of Biology, Faculty of Science, Tohoku University, Aramaki, Aoba-Ku, Sendai, 980, Japan;(4) Attic Lab, Komrgafukuro 1-6-2, Aoba-Ku, Sendai, 980, Japan
Abstract:Recent studies on the colony formation of soil bacteria opened the way to categorize soil bacteria into colony forming curve (CFC) groups of different growth rates. A bacterial culture collection comprising organisms from every CFC group is called an ecocollection. Outlines of ECs of paddy soil 1992 and grassland soil 1987 and 1992 were described. Phylogenetic studies by 16S rDNA sequencing showed a great diversity of culture strains of the ecocollections (EC). A set of alternative concepts was proposed; the active and the quiescent forms of bacterial cells in soil. The former is able to be cultivated and thus counted by the plate method, while the latter is not unless it transforms into the former. Based on the results several points required for extensive cataloguing of soil bacteria were noted.
Keywords:colony formation  viable count  culture collection
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