Proteomic analysis of human epithelial ovarian cancer xenografts in immunodeficient mice exposed to chronic psychological stress |
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Authors: | Jun Gao GuoLan Gao YanYu Zhang Fen Wang |
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Institution: | (1) Department of Naval Medicine, Second Military Medical University, Shanghai, 200433, China;(2) Department of Traditional Chinese Medicine, Changhai Hspital, Shanghai, China; |
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Abstract: | This work aims at comparing alterations in the proteomes of human epithelial ovarian cancer xenografts between stressed and
non-stressed immunodeficient mice as well as exploring the molecular mechanisms linking chronic psychological stress to ovarian
cancer oncogenesis and progression. SK-OV-3 cells were injected subcutaneously into nude mice. The stress group was subjected
to a chronic physical restraint protocol for 6 h on 35 consecutive days, while the control group was unrestrained. All mice
were sacrificed on day 36 after SK-OV-3 cell injection, and tumors were excised. Tumor tissues were processed for 2D gel electrophoresis,
mass spectrometry (nanoUPLC-ESI-MS/MS) and Western blotting. The expression of 20 proteins was found to be significantly altered
between the stress and control groups, of which 14 were up-regulated, five were down-regulated, and one protein was found
only in the stress group. All proteins were identified by UPLC-ESI-MS/MS, and Western blotting results were consistent with
those of proteomic methods. The present results provide new evidence relating to the molecular mechanism underlying the relationship
between psychological stress and tumor progression. |
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Keywords: | |
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