蚕豆叶片细胞中IAA的胶体金免疫电镜定位

利用胶体金免疫电镜技术对蚕豆(Vicia faba L.)叶片细胞中的IAA定位进行了研究。幼嫩叶片的叶肉细胞中金颗粒主要分布在细胞核和叶绿体中,细胞质及细胞壁也有金颗粒标记。成熟叶片的叶肉细胞中金颗粒主要分布在叶绿体和细胞质,细胞壁也有少量金颗粒标记,液泡中没有发现金颗粒标记。成熟叶片小叶脉的韧皮细胞发现有大量的金颗粒标记,金颗粒主要标记在传递细胞的细胞壁中。小叶脉的维管束鞘细胞中也有很多的金颗粒标记,金颗粒主要分布在叶绿体、细胞质及细胞壁中。幼嫩叶片组织不进行IAA的固定或用正常兔IgG代替IAA抗体染色的对照,很难发现金颗粒标记。对IAA在组织及亚细胞中的定位及其生理意义进行了讨论。

IMMUNO-GOLD LOCALIZATION OF IAA IN THE LEAF CELLS OF VICIA FABA

Subeellular localization of IAA in the leaf cells of Vicia faba L. was studied with im-muno-gold electron microscopic technique. Nuclei and chloroplasts in mesophyll cells of the immature leaves were labeled with numerous gold particles, which were also seen in the cytoplasm and cell wall, In mesophyll cells of the mature leaves, gold particles were mainly distributed over the chloroplasts and cytoplasm, and a few were also located in the cell wall; but, however, none was seen in the vacuoles. Many gold particles were distributed over the phloem cells of the fine veins of the mature leaves, and were mainly located in the cell wall of the transfer cells. Some were also located in the cells of vascular bundle sheath of the fine veins, mainly distributed over the chloroplasts, cytoplasm and cell wall. Sections of mesophyll cells of the immature leaves without EDC (1-ethyl-3(3-dimethylamino-propyl) carbodiimide) fixation or treated with normal rabbit IgG instead of anti-IAA rabbit serum were devoid of gold particle label. Tissue and subcellular immuno-localization of IAA and its physiological significances were discussed.