Adherent cell requirement for PWM-stimulated factor suppression of natural killer cytotoxicity |
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Authors: | F Hofman N Rand J L Fahey |
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Institution: | Department of Microbiology and Immunology, University of California School of Medicine, Los Angeles, California 90024 U.S.A. |
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Abstract: | Natural killer cytotoxicity is enhanced in a 5-day mixed-leukocyte culture (MLC). This augmentation of NK can be suppressed by factor released from pokeweed-mitogen (PWM)-activated peripheral blood cells. To maximize activity, the PWM-soluble factor must be present within the first 6 hr of the MLC, and is required to be in the culture for at least 4 hr. The affect of the PWM factor is not due to the destruction of either effector or stimulator cells. This factor does, however, require the presence of an adherent, non-T cell in the effector population. Directly incubating adherent cells with the PWM-induced soluble factor activates this population to mediate suppression. Thus the adherent cells are required for the PWM-induced suppression of NK cytotoxicity, indicating a possible regulatory mechanism for this cytotoxicity. |
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