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Induction of somatic embryogenesis and plantlets in tendrils of Vitis vinifera L.
Authors:C. K. Salunkhe  P. S. Rao  Minal Mhatre
Affiliation:(1) Biotechnology Division and L & CM Section, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India Fax no.: +091-22-556-0750 E-mail: biotech@magnum.barc.ernet.in, IN
Abstract:Somatic embryogenesis was observed in callus initiated from tendril explants of Vitis vinifera L. cvs. Thompson, Sonaka and Tas-e-Ganesh on Emershad and Ramming medium supplemented with 1 μm 6-benzylaminopurine. Low-frequency conversion to shoots was obtained in the third and fourth subculture on the same medium. Emerging shoots subsequently formed complete plantlets on liquid rooting medium containing 1 μm indole-3-acetic acid. The possible use of tendrils as a novel explant for somatic embryogenesis in grape is discussed. Received: 3 March 1997 / Revision received: 21 May 1997 / Accepted: 25 June 1997
Keywords:Vitis vinifera  Tendrils  Somatic embryos  Plantlets
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