| Institution: | (1) National Horticultural Research Institute, RDA, 440-706 Suwon, Korea;(2) Department of Horticultural Science, Cheju National University, 690-756 Cheju, Korea;(3) College of Agriculture and Life Sciences, Kangwon National University, 200-701 Chuncheon, Korea;(4) Department of Applied Life Science, College of Life and Environment Science, Konkuk University, 143-701 HwaYangDong, KwangJinKu, Seoul, Korea |
| Abstract: | In Fuji, the production of ethylene was increased with the addition of AgNO3 and inhibited with the addition of 10  M aminoethoxyvinylglycine (AVG). The addition of 80 M AgNO3 to transformed explants of  Fuji cultured on selection medium resulted in increased ethylene production (20 l l–1) at 3 weeks. Under examining the effect of AgNO3 in Fuji, the 40 M AgNO3 showed with higher 33.8% and 6.5% in the efficiency of regeneration and transformation. However, ethylene production in Gala explants treated with 10M AgNO3 (3 l l–1) decreased after 2 weeks compared with the control (5 l l–1). Although the regeneration efficiency of Gala with 10 M AgNO3 was higher (41.1%) than the control (20.1%), there was no significant difference in the transformation efficiency at the same concentration. Shoot regeneration of Fuji and Gala was completely inhibited with 10 M AVG. These results suggest that the addition of AgNO3 affects the efficiency of Agrobacterium-mediated gene transfer in Fuji.Eun Soo Seong, Ill Min Chung- These two Authors Contributed equally to this work |
