昆虫细胞（Sf21）悬浮培养过程中生长限制性基质间歇补加技术的 …

基于ｒ２１昆虫细胞在浮过程中所表现出的生长代谢特征,提出以培养液中残糖浓度作为控制参数,并利用限制性基质（葡萄糖和蛋白水解物）的间歇补加技术调控细胞生长的方案。实际控制表明：与批培养相比,Ｓ１ｆ２１细胞在两种具代表性的昆虫水解物）的间歇补加调控细胞生长的方案。实际控制表明：与批培养相比,Ｓｆ２１细胞在两种具代表性的昆虫细胞培养基（ＩＰＬ－４１和ＴＣ－１００）中的生长期和稳定期部都到有效的延长。ＴＣ

Application of Intermittent-feeding of Growth-limiting Nutrients in SuspensionCulture of Insect Cells(Sf21)

On the basis of the growth and metabolism behavior inherent in suspended Sf21 insect cells, the intermittent feeding of growth-limiting nutrients(glucose and protein hydrolysates) was employed in the regulation of cellular growth during the cultivation by using the residual glucose concentration as a reference point. It was shown that as compared with the batch cultivation, the intermittent-feeding of growth-limiting nutrients effectively prolonged the growth and stationary phase for Sf21 insect cells grown in two representative culture medium(TC-100 and IPL-41). The maximum cell density was increased from 3.0 x 10(6) cells/ml to 6.5 x 10(6) cells/mL in TC-100 medium, and in IPL-41 medium, the maximum cell density was increased from 7.05 x 10(6) cells/mL to 9.0 x 10(6) cells/mL. As the defined nutrient solution was used for feeding in lieu of the complicated and expensive base medium, the technique would find prospects in large scale high-density cultivation of insect cells.