In vitro regeneration of Gaillardia pulchella Foug. |
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Authors: | J E Bourque S Tanner T J Mabry |
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Institution: | (1) Department of Botany, University of Texas, 78713 Austin, Texas, USA |
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Abstract: | Young leaf and internodal stem segments of Gaillardia pulchella, collected from wild species re-established in the greenhouse, were used to initiate callus on Murashige & Skoog medium supplemented
with NAA (2.0 mgl−1) and BA (0.4 mgl−1). Callus formed after 10 to 14 days in the dark. Cultures were transferred to fresh medium and placed under lighted conditions
where shoot formation occurred approximately 14 to 30 days after initiation. Callus sub-cultured at 14 to 21-day intervals
continued to produce primordia for several weeks. Flowers were produced by regenerated shoots maintained on MS medium, but
roots did not develop until the plantlets were transferred to soil conditions. |
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Keywords: | Gaillardia in vitro regeneration organogenesis tissue culture |
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