The 9-Fluorenylmethoxycarbonyl (Fmoc) Group and Its Use in Oligonucleotide Synthesis |
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| Authors: | H. Schirmeister-tichy G. G. Alvarado W. Pfleiderer |
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| Affiliation: | 1. Fakult?t für Chemie, Universit?t Konstanz , Postfach 5560, D-78434, Konstanz;2. Dept. of Biochemistry , University of Ottawa , Vachon Hall 202, 40 Marie Curie Priv., Ottawa, Ontario, Canada , K1N6N5 |
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| Abstract: | Abstract The introduction of the base-labile 9-fluorenylmethoxycarbonyl (Fmoc) group into the exocyclic amino function of 2′-deoxynucleosides and their dimethoxytritylation and phosphitylation is described. The resulting key intermediates were investigated in the built-up of different oligodeoxyribonucleoside phosphate and thiophosphate chains which were deprotected under mild basic conditions leading to crude oligomers of high purity. |
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