Studies on the Interaction of Dna-Ligands with Modified Parallel-Stranded Duplex-Dna Oligomers |
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| Authors: | I Förtsch E Birch-Hirschfeld T M Jovin A Stelzner C Zimmer |
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| Institution: | 1. Institute of Molecular Biology, Max Planck Institute for Biophysical Chemistry , D-37070 , G?ttingen , Germany;2. Institute of Virology, Friedrich Schiller University Jena , Winzerlaer Str. 10, D-07745 , Jena , Germany;3. Department of Molecular Biology , Max Planck Institute for Biophysical Chemistry , D-37070 , G?ttingen , Germany |
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| Abstract: | Abstract We have investigated the minor groove binders netropsin (Nt) and related lexitropsins for possible interactions with parallel-stranded DNA (ps-DNA). The fluorescence emission spectra and their temperature dependence between 4°C and 30°C led to two conclusions: (i) The specific ligand Nt induces a conversion of the ps-DNA to an antiparallel-stranded DNA (aps-DNA) with mismatched base pairs, a reaction which is much less pronounced for the imidazole-containing analogs, (ii) The more weakly binding imidazole-bearing netropsin-analogs may bind to ps-DNA. |
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