Tetraethylene Glycol-Derived Spacer for Oligonucleotide Synthesis |
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Authors: | Sigmund Gunzenhauser Ewa Biala Peter Strazewski |
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Affiliation: | Institute of Organic Chemistry, University of Basel , St. Johanns-Ring 19, CH, 4056, Basel, Switzerland |
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Abstract: | Abstract 3,6,9-Trioxaundecane-1,11-diisocyanate (6) was synthesised from tetraethylene glycol in 5 steps and 48 % overall yield. Spacer 6 was monofunctionalised with the fully protected adenosyl-3′-O-succinate derivative 7 and linked to aminomethyl polystyrene affording a solid support suitable for oligoribonucleotide synthesis (loading: ~20 μmol/g). The HPLC analysis of a crude oligoribonucleotide synthesis and the yield of the full-length product show that this spacer compares well to hexamethylene diamine. |
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