Studies Towards the Synthesis of Peptide-Oligonucleotide Conjugates |
| |
Authors: | Suzanne Peyrottes Béatrice Mestre Fabienne Burlina Michael J Gait |
| |
Institution: | Medical Research Council, Laboratory of Molecular Biology , Hills Road, Cambridge, CB2 2QH, U.K. |
| |
Abstract: | Abstract We describe a peptide fragment, solid-phase coupling strategy for synthesis of peptide-oligonucleotide conjugates. Model conjugates contained a hydrophobic tetrapeptide, a hydrophobic influenza virus fusion nonapeptide, or a basic octapeptide of the HIV-1 Tat protein coupled to either dT12 or a 16-mer anti-Tat oligodeoxyribonucleotide. Conjugation yields were improved by removal of internucleotide 2-cyanoethyl groups prior to peptide coupling and by use of a C12 spacer between peptide and oligonucleotide. |
| |
Keywords: | |
|
|