利用脱落酸和冷适应提高坛紫菜丝状体种质的冻存效率

为验证脱落酸(ABA)和冷适应能否提高紫菜丝状体种质的冷冻保存效率,研究以坛紫菜(Pyropia haitanensis)自由丝状体为材料,在20℃,光强40μmol·photons/(m2·s),在光周期12L﹕12D条件下以ABA(100μmol/L)处理48h或4℃暗培养6d,随后进行–80℃冷冻保存实验.通过连...

THE ROLE OF ABSCISIC ACID AND COLD ADAPTATION IN IMPROVING THE CRYOPRESERVATION EFFICIENCY OF PYROPIA HAITANENSIS CONCHOCELIS

To explore whether abscisic acid(ABA)and cold adaptation can improve the cryopreservation efficiency of Porphyra sensu lato,the free living conchocelis of Pyropia haitanensis were treated with ABA(100μmol/L)for 48h at 20℃,light intensity of 40μmol·photons/(m²·s)with photoperiod 12L﹕12D or cultured in dark at 4℃for 6 days before cryopreservation at-80℃.The effects of cryopreservation were evaluated by continuous measurement of algal survival rate after frozen,the maximum light quantum efficiency(F_v/F_m)of PSⅡand the expression of cold adaptative related genes,such as P(H⁺)-ATPase,ald,Mn-sod A,Cu/Zn-sod 1,apx-2,hsp70-4,psy-2,gapdh,and gpxha-1.The results showed that:(1)Both ABA treatment and cold adaptation at 4℃increased the survival rate of P.haitanensis conchocelis after cryopreservation,59%in the ABA groups and 89%in the cold adapt groups;(2)The F_v/F_mof the conchocelis after cold adaptation recovered significantly faster than those of the other groups(P<0.05),with the optimal level after 35 day culture;(3)ABA treatment and 4℃cold adaptation significantly up-regulated genes like P(H⁺)-ATPase,ald,Mn-sod A,Cu/Zn-sod 1,gapdh(P<0.01)and psy-2(P<0.05)in the algae,but only ABA induced apx-2 and hsp70-4(P<0.01).In summary,ABA treatment and cold adaptation can improve the frost resistance of P.haitanensis and improve the efficiency of cryopreservation.The cold adaptation method at 4℃is simple and effective,and has greater application potential.