Identification of aryl-phospho-β-<Emphasis Type="SmallCaps">d</Emphasis>-glucosidases in<Emphasis Type="Italic"> Bacillus subtilis</Emphasis> |
| |
Authors: | Barbara?Setlow Arturo?Cabrera-Hernandez Rosa?Martha?Cabrera-Martinez Email author" target="_blank">Peter?SetlowEmail author |
| |
Institution: | (1) Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA;(2) Departamento de Bioquimica, Instituto Politecnico Nacional, Escuela Nacional de Ciencas Biologicas, 11340 Mexico City, Mexico;(3) Present address: Center for Immunotherapy, University of Connecticut Health Center, Farmington, CT 06032, USA |
| |
Abstract: | Four aryl-phospho--d-glucosidases were identified in Bacillus subtilis by using 4-methylumbelliferyl-phospho--d-glucopyranoside as a substrate. Two of these enzymes are the products of the bglA and bglH genes, previously suggested to encode aryl-phospho--d-glucosidases, while the other enzymes are encoded by the yckE and ydhP genes. Together, these four genes account for >99.9% of the glucosidase activity in B. subtilis on aryl-phospho--d-glucosides. yckE was expressed at a low and constant level during growth, sporulation, and spore germination, and was not induced by aryl--d-glucosides. ydhP was also not induced by aryl--d-glucosides. However, while ydhP was expressed at only a very low level in exponential-phase cells and germinating spores, this gene was expressed at a higher levels upon entry into the stationary phase of growth. Strains lacking yckE or ydhP exhibited no defects in growth, sporulation, or spore germination or in growth on aryl--d-glucosides. However, a strain lacking bglA, bglH and yckE grew poorly if at all on aryl--d-glucosides as the sole carbon source.Abbreviations
MU
4-Methylumbelliferone
-
MUG
4-Methylumbelliferyl--d-glucopyranoside
-
MUGal
4-Methylumbelliferyl--d-galactopyranoside
-
MUG-P
4-Methylumbelliferyl--d-glucopyranoside-6-phosphate |
| |
Keywords: | Bacillus subtilis bglA bglH yckE ydhP Phosphoglucosidase Arylglucosides |
本文献已被 PubMed SpringerLink 等数据库收录! |
|