杀雄剂SQ-1诱导小麦雄性不育花粉粒差异蛋白质组学研究

采用固相pH梯度/SDS-PAGE双向凝胶电泳对经杀雄剂SQ-1处理和未处理的小麦(Triticum aestivum)成熟期花粉总蛋白质进行了分离, 银染显色, 获得了分辨率和重复性较好的双向电泳图谱. 通过PDQuest 2DE图像软件的分析, 在等电点4～7之间可识别350个以上较为清晰的蛋白质点, 其中差异表达明显的蛋白质点数为21个. 将11个差异点采用基质辅助激光解析电离飞行时间质谱进行了肽质量指纹图谱分析, 采用Mascot软件在Swiss-prot数据库查询, 鉴定出了7个蛋白质, 它们分别是液泡转化酶、动力蛋白轻链TCTEX-1、锰超氧化物歧化酶、果糖-1,6-二磷酸醛缩酶、抗坏血酸过氧化物酶、凝集素蛋白激酶和一种未知功能的蛋白. 对已知蛋白的功能进行分析, 推测杀雄剂SQ-1诱导小麦雄性不育可能与能量代谢失衡、淀粉合成受抑制、活性氧积累、细胞凋亡以及花器官发育调节基因作用失控等有关.

Differential Proteomic Studies on Pollen Grain Proteins of Wheat Male Sterile Line Induced by Chemical Hybridizing Agent SQ-1

The pollen grain proteins in maturation-stage from wheat (Triticum aestivum) male sterile line induced by chemical hybridizing agent SQ-1 were separated by 2-D electrophoresis with immobilized pH (4～7) gradients as first dimension and SDS-PAGE as second dimension. The silver-stained protein spots were analyzed using PDQuest software, over 350 protein spots were detected reproducibly within pI 4～7 on each 2-Dgel. Twenty-one spots were found to be differentially expressed. Seven differentially expressed protein spots out of 11 were identified by MALDI-TOF mass spectrometry analysis and protein database searching, which are vacuolar invertase, ascorbate peroxidase, putative fructose-1,6-biphosphate aldolase, dynein light chain TCTEX-1,lectin protein kinase, manganese superoxide dismutase and predicted protein. The functions of major differentially expressed proteins were reviewed and discussed. It was inferred that the male sterility induced by SQ-1 might related to energy metabolism turbulence,starch synthesis suffocation, active oxygen accumulation, apoptosis and disturbed function of the flower developmental gene.