In vitro induction of inflorescence in <Emphasis Type="Italic">Dioscorea</Emphasis><Emphasis Type="Italic">zingiberensis</Emphasis> |
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Authors: | Xiao-Long Huang Bo Yang Chun-Gen Hu Jia-Ling Yao |
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Institution: | (1) College of Life Science and Technology, Huazhong Agricultural University, 430070 WuHan, Hubei, China;(2) College of Horticulture and Forestry Science, Huazhong Agricultural University, 430070 WuHan, Hubei, China; |
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Abstract: | Inflorescence induction and morphogenesis of regenerated flowers were investigated in vitro in Dioscorea zingiberensis C. H. Wright. Inflorescence induction was influenced by the type and concentration of phytohormones. When floral bud explants
were incubated on a Murashige and Skoog medium containing a combination of 2.0 mg l−1 6-benzyladenine and 0.5 mg l−1 indole-3-butyric acid, the highest frequency of inflorescence induction was observed. However, in the presence of gibberellic
acid, induction efficiency was reduced although node length of inflorescence was increased. Ontogenetic studies revealed that
the inflorescence primordia originated directly from axillary epidermal cells of the perianth and bract of the explants after
7 days. In vitro, male flowers developed normally and blossomed after 90–100 days. In addition, some bisexual flowers were
observed. These results demonstrated that there were differences in sexual differentiation of floral buds in vitro compared
with that in vivo. |
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