Renal cell cultures for the study of growth factor interactions underlying kidney organogenesis |
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Authors: | Letizia Mattii Francesco Bianchi Iana Da Prato Amelio Dolfi Nunzia Bernardini |
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Institution: | (1) Department of Human Morphology and Applied Biology, Section of Histology and General Embryology, Faculty of Medicine and Surgery, Pisa University, Via Roma 55, 56126 Pisa, Italy |
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Abstract: | Summary The present study was performed in four renal cell lines to evaluate their capability to: (1) produce and express transforming
growth factor α (TGFα), its respective receptor, the epidermal growth factor receptor (EGFr) and the small G protein, RhoA,
and (2) exhibit morphogenetic properties when grown on Matri-cell substrates. The cell lines were derived from normal (Madin-Darby
canine kidney cells), embryonic (SK-NEP-1 and 293 cells), and cancerous (human renal adenocarcinoma cells) kidneys. TGFα messenger
ribonucleic acid evaluated by a nonradioactive in situ hybridization technique, was found to be expressed in all the cell
lines. Large amounts of TGFα peptide were observed in all four cell lines, while EGFr was highly expressed only in cancerous
ACHN and embryonic-tumor SK-NEP-1 cells. RhoA peptide was found in appreciable amounts in SK-NEP-1 and 293 cells (compared
to the other two cell lines). The morphogenetic properties of the four cell lines were assessed, by culturing them on Matri-cell
dishes: SK-NEP-1 cells alone were found to grow in three-dimensional structures forming clusters and worm-like cellular aggregates.
This feature was displayed by SK-NEP-1 cells but not by the other three cell lines, and may be connected with the contemporary
presence of RhoA, EGFr, and TGFα found in significant amounts only in the SK-NEP-1 cell line. |
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Keywords: | TGFα /EGFr RhoA kidney cell lines morphogenetic properties on Matri-cell |
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