Cell surface glycoproteomic analysis of prostate cancer-derived PC-3 cells |
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Authors: | Hubbard Sarah C Boyce Michael McVaugh Cheryl T Peehl Donna M Bertozzi Carolyn R |
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Institution: | Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA. |
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Abstract: | Most clinically approved biomarkers of cancer are glycoproteins, and those residing on the cell surface are of particular interest in biotherapeutics. We report a method for selective labeling, affinity enrichment, and identification of cell-surface glycoproteins. PC-3 cells and primary human prostate cancer tissue were treated with peracetylated N-azidoacetylgalactosamine, resulting in metabolic labeling of cell surface glycans with the azidosugar. We used mass spectrometry to identify over 70 cell surface glycoproteins and biochemically validated CD146 and integrin beta-4, both of which are known to promote metastatic behavior. These results establish cell-surface glycoproteomics as an effective technique for discovery of cancer biomarkers. |
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Keywords: | Metabolic labeling Glycoproteomics Prostate cancer Azidosugar Tissue slice cultures |
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