Plant regeneration from callus and protoplast cultures of Helianthus giganteus L. |
| |
Authors: | Sergei Krasnyanski Zsolt Polgár Gizella Németh László Menczel |
| |
Institution: | (1) Institute of Plant Physiology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701 Szeged, Hungary;(2) Cereal Research Institute, POB 391, H-6701 Szeged, Hungary |
| |
Abstract: | Summary Methods of plant regeneration from callus and protoplasts of Helianthus giganteus L. are described. Embryogenic callus was obtained from leaf explants and plants were regenerated from these calli on MS media with different combinations of benzyladenine and naphtaleneacetic acid. Leaf protoplasts isolated from in vitro grown plants formed somatic embryos when cultured in agarose solidified droplets of V-KM medium containing benzyladenine and naphtaleneacetic acid. Embryos developed into plantlets on media with reduced auxin contents. Regenerated plants were successfully planted in soil.Abbreviations BA
benzyladenine
- IAA
indoleacetic acid
- MS
Murashige and Skoog medium
- NAA
naphtaleneacetic acid
- V-KM
protoplast culture medium of Binding and Nehls |
| |
Keywords: | Helianthus giganteus Protoplasts Plant regeneration |
本文献已被 SpringerLink 等数据库收录! |