Polyploidy and aspartate-transcarbamylase activity in Hippocrepis comosa L. |
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Authors: | Monique Guern Guy Hervé |
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Institution: | (1) Laboratoire de Taxonomie Expérimentale et Numérique de la Faculté des Sciences, associé au C.N.R.S., Bât. 362, F-01405 Orsay, France;(2) Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique, F-91190 Gif-sur-Yvette, France |
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Abstract: | The DNA content of plants which were sampled in natural di-, tetra- and hexaploid populations of Hippocrepis comosa L. was estimated and the aspartate transcarbamylase activities of the corresponding cell-free extracts were compared. The amount of DNA is not exactly proportional to the number of genomes. The three kinds of populations do not differ in their aspartate transcarbamylase specific activity. While the enzyme properties are identical in the extracts derived from the diploid and hexaploid plants, the aspartate transcarbamylase present in the tetraploid cytotype shows a slightly lower affinity for one of its substrates and a significantly lower sensitivity to the feedback inhibitor UTP which is still observed after partial purification. These properties might be related to the previously reported greater ability of the tetraploid cytotype to adapt to a variety of biotopes.Abbreviations ATCase
aspartate transcarbamylase
- CAP
carbamylphosphate
- EDTA
ethylenediaminetetracetic acid
- Tris
trihydroxymethylaminomethane
- AMP
adenosine monophosphate
- ATP
adenosine triphosphate
- CMP
cytidine monophosphate
- CTP
cytidine triphosphate
- UMP
uridine monophosphate
- UTP
uridine triphosphate |
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Keywords: | Aspartate transcarbamylase Hippocrepis Polyploidy |
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