Multilocus DNA fingerprints in the plant Cynoglossum officinale L. and their use in the estimation of selfing |
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| Authors: | K. Vrieling,P. Saumitou-Laprade,E. Meelis,& J. T. Epplen |
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| Affiliation: | Laboratoire de Génétique et Évolution des Populations Végétales, URA CNRS 1185, Universitéde Lille, Bâtiment SN2, F-59655 Villeneuve d'Ascq Cedex, France,;Institute of Evolutionary and Ecological Sciences, Van der Klaauw Laboratory, PO Box 9516, 2300 RA Leiden, the Netherlands,;Molecular Human Genetics, Ruhr-Universität, W-44780 Bochum, Germany |
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| Abstract: | We have developed a nonradioactive oligonucleotide multilocus DNA fingerprinting method for Cynoglossum officinale . Of the 19 probes tested, six probes yielded banding patterns for all restriction enzymes used. All but one of the informative probes are repeats with a four-base motif. Approximately 60% of the loci appeared to be polymorphic. The sensitivity of the nonradioactive method was equal to that of the radioactive method. In addition, a new simple calculation method is presented to estimate selfing rates and approximate 95% confidence limits from the DNA fingerprint profiles avoiding 'between-gel' comparisons. The selfing rates differed significantly (as determined from 95% confidence intervals) between naturally pollinated individuals of C. officinale within the experimental population. The estimates ranged from 0 to 70% selfing. |
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| Keywords: | multilocus DNA fingerprinting (GATA)4 synthetic oligonucleotide probes Cynoglossum officinale Hound's-Tongue |
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