|
|||||
|
|
| 叶螨线粒体COI基因中央区段的PCR扩增 | |
| 引用本文： | 刘天祥 刁兆彦. 叶螨线粒体COI基因中央区段的PCR扩增[J]. 蛛形学报, 1998, 7(2): 96-102 |
| 作者姓名： | 刘天祥 刁兆彦 |
| 作者单位： | 复旦大学环境与资源生物系，复旦大学环境与资源生物系，复旦大学环境与资源生物系 上海 200433，上海 200433，上海 200433 |
| 基金项目： | 国家教委高等学校博士点基金 |
| 摘 要： | 根据二点叶螨线粒体ＣＯＩ基因序列设计１对ＰＣＲ引物，对叶螨科不同种属的线粒体ＣＯＩ基因中央区段进行了ＰＣＲ扩增，结果表明该对引物能成功扩增叶螨科７属９种叶螨的约３４０ｂｐ的同源片段，由于叶螨ＤＮＡ序列资料非常有限，扩大引物的使用范围成为快速获得物特定基因序列的有效途径，研究结果使根据叶螨线粒体ＣＯＩ基因序列信息探索其系统演化成为可能，另外，还对模板ＤＮＡ分离方法进行了优化。 |
| 关 键 词： | 叶螨 线粒体 PCR COI基因 |
PCR AMPLIFICATION OF MI TOCHONDRIAL COI GENE CENTRAL REGION OF SPIDER MITES IN FAMILY TETRANYCHIDAE (ACARI: TETRAI YCHOILEA) |
|
| Lin Tianxiang,E ao Zhac ''an and Dong Huiqin. PCR AMPLIFICATION OF MI TOCHONDRIAL COI GENE CENTRAL REGION OF SPIDER MITES IN FAMILY TETRANYCHIDAE (ACARI: TETRAI YCHOILEA)[J]. Acta Arachnologica Sinica, 1998, 7(2): 96-102 | |
| Authors: | Lin Tianxiang E ao Zhac ''an Dong Huiqin |
| Abstract: | A ] air of PCR pri ners flanking central region of mitochondrial cy-tochrome oxidase (COI) subunit Igene of spider mite (Tetranychus urticae) were designed and used to a mplify homologous fragments from nine mite species in seven genera of family Tetranych dae. Successful mplifications of expected about 340 base pair fragments have been ac rieved for all n rterials used. Since very limited sequence data are available in mites, i is an effective pproach to broaden utilization range of primers in order to retrieve ger: sequences. Out trial makes it possible to explore phylogenetic system of mites according to their mtD 4A COI gene sequence information. In addition,extraction method of t mplate DNA w s optimized in this study. |
| Keywords: | Lonf mite Mitoc] ondrial cytochrome oxidase subunit I gene PCR |
| 本文献已被 CNKI 维普 等数据库收录！ | |