Vitamin B12 production by isopropanol-assimilating microorganisms |
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| Affiliation: | 1. Laboratoire de Physico-chimie des Matériaux, Faculté des Sciences de Monastir, Avenue de l''environnement, 5019 Monastir, University of Monastir, Tunisia;2. Centre de Diffractométrie X, Institut des Sciences Chimiques de Rennes, UMR 6226, CNRS–Université de Rennes, 1, Campus de Beaulieu, 35042 Rennes Cedex, France;3. Département de Chimie Moléculaire, Université Grenoble-Alpes, CNRS UMR 5250, BP53 38041 Grenoble, France;1. College of Food Science and Engineering, Wuhan Polytechnic University, 68 Xuefu South Road, Wuhan, Hubei 430023, PR China;2. Wuhan Institute of Virology, Chinese Academy of Sciences, 44 Xiaohongshan, Wuhan, Hubei 430071, PR China;1. AMC Engineering College, Bannerghatta Main Rd, Bengaluru, Karnataka, 560083, India;2. School of Electrical & Electronics Engineering, SASTRA Deemed University, Tirumalaisamudram, Thanjavur, 613 401, India;1. State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;2. Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China;3. Department of Chemistry, Shanghai Key Lab of Chemical Assessment and Sustainability, Tongji University, Shanghai 200092, China;1. Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Science, Wuhan University, Wuhan, Hubei 430072, PR China;2. Wuhan University Suzhou Research Institute, Suzhou, Jiangsu 215123, PR China |
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| Abstract: | ![]()
We isolated about 500 isopropanol(IPA)-assimilating bacteria from many soil samples, among which 23 strains produced vitamin B12. Taxonomical studies of the best producer, designated strain Hi16.3, showed that it belonged to the genus Arthrobacter. Vitamin B12 production by the strain was higher than that by 12 other authentic Arthrobacter spp. using glucose as a sole carbon source. In fed-batch culture, the maximum production yield with strain Hi16.3 (named A. hyalinus) was 2 mg/l in the culture broth, when 80 ml of IPA/l broth was consumed. |
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