A Simple Method for the Mating of Sclerotinia trifoliorum |
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Affiliation: | 1. Animal Breeding & Genomics and Animal Behaviour & Welfare Teams, Animal and Veterinary Sciences Group, SRUC (Scotland''s Rural College), West Mains Road, Edinburgh EH9 3JG, United Kingdom;2. The Roslin Institute, R(D)SVS, University of Edinburgh, Division of Genetics and Genomics, Easter Bush, Midlothian EH25 9RG, United Kingdom;3. PIC Europe, Alpha Building, London Road, Nantwich CW5 7JW, United Kingdom;1. Department of Biology, Acadia University, 33 Westwood Avenue, Wolfville, Nova Scotia B4P 2R6, Canada;2. U.S. Department of Energy Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA;3. Department of Plant and Microbial Biology, UC Berkeley, 111 Koshland Hall, Berkeley, California 94720, USA;1. Departamento de Biologia, CESAM, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;2. Department of Land, Environment, Agriculture, and Forestry (TeSAF), University of Padova, Viale dell''Università 16, 35020, Legnaro, PD, Italy;3. University of Lisbon, Faculty of Sciences, Biosystems and Integrative Sciences Institute (BioISI), Campo Grande, 1749-016, Lisbon, Portugal;1. School of BioSciences, University of Melbourne, VIC 3010, Australia;2. Department of Biology, Amherst College, Amherst, MA 01002, USA;3. Department of Organismal Biology, Uppsala University, Norbyvägen 18D, 752 36 Uppsala, Sweden;4. Laboratoire Ecologie, Systématique et Evolution, UMR 8079, Bâtiment 360, Univ Paris Sud, 91405 Orsay, France;5. CNRS, 91405 Orsay, France;1. Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum national d''Histoire naturelle, CNRS, Sorbonne Université, EPHE. CP 75005, 57 rue Cuvier, 75005 Paris, France;2. Département de Biologie, Ecole Normale Supérieure, PSL Research University, 75005 Paris, France;3. Facultad de Agronomía, Universidad de Buenos Aires, Capital Federal, Argentina |
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Abstract: | Rehnstrom, A. L., and Free, S. J. 1993. A simple method for the mating of Sclerotinia trifoliorum. Experimental Mycology 17, 236-239. A simple method which allows for the controlled mating of L and S mating type strains of Sclerotinia trifoliorum is described. Using the method, we have been able to mate L and S strains and have demonstrated the segregation of the genetic markers involved in mycelial incompatibility into the progeny. |
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