Clonal nature of murine hemopoietic blast cell colonies |
| |
| Authors: | Y Fujimori H Hara T Taguchi Y Kitamura K Nagai |
| |
| Institution: | Second Department of Internal Medicine, Hyogo College of Medicine, Japan. |
| |
| Abstract: | Murine hemopoietic blast cell colonies obtained from spleen cells of 5-fluorouracil (5-FU)-treated mice give rise to many multilineage colonies including granulocyte - erythrocyte - macrophage - megakaryocyte (GEMM) colonies in secondary cultures. Progenitor cells for blast cell colonies are considered to be more primitive than colony forming units (CFU)-GEMM. To determine whether they are clonal, we examined the phosphoglycerate kinase-1 (PGK-1) isozyme type of colonies originally grown from spleen cells of 5-FU-treated mice which had PGK-1 isozyme mosaicism. PGK assays of whole secondary colonies derived from one blast cell colony showed that they were either of type A or type B but not both. These results suggest that murine hemopoietic blast cell colonies are clonal. |
| |
| Keywords: | |
|
|
