The delta-endotoxin of Bacillus thuringiensis. V. On the occurrence of endotoxin fragments in hemolymph |
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| Authors: | P G Fast E Videnova |
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| Institution: | Insect Pathology Research Institute, Canadian Forestry Service, Environment Canada, P.O. Box 490, Sault Ste. Marie, Ontario, Canada;Plant Protection Institute, Sofia 9, Kostinbrad, Bulgaria |
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| Abstract: | Leucine-3H labeled crystals of Bacillus thuringiensis δ-endotoxin were fed to last-instar larvae of spruce budworm, Choristoneura fumiferana, eastern forest tent caterpillar, Malacosoma disstria, and silkworm, Bombyx mori. Radioactivity was detected in hemolymph 1 min after feeding in the first two species, but not until 3–5 min after feeding in silkworm larvae. Most of the radioactivity from hemolymph of all three species eluted from gel filtration columns at the same elution volume indicating similar moleculear weights (<1800 daltons). |
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