Relation between phylogenetic position, lipid metabolism and butyrate production by different Butyrivibrio-like bacteria from the rumen |
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Authors: | Delphine Paillard Nest McKain Lal C Chaudhary Nicola D Walker Florian Pizette Ingrid Koppova Neil R McEwan Jan Kope?ný Philip E Vercoe Petra Louis R John Wallace |
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Institution: | (1) Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB, UK;(2) Present address: Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute, Izatnagar, 243 122, India;(3) Present address: Unité de Microbiologie, INRA de Clermont-Ferrand-Theix, 63122 St-Genès Champanelle, France;(4) Present address: Institute of Animal Physiology & Genetics, Videnska 1083, 142 20 Prague 4, Krc, Czech Republic;(5) Present address: Institute of Rural Science, University of Wales, Llanbadarn Campus, Aberystwyth, SY23 3AL, UK;(6) Present address: Animal Science Group, University of Western Australia, Crawley, WA, 6009, Australia |
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Abstract: | The Butyrivibrio group comprises Butyrivibrio fibrisolvens and related Gram-positive bacteria isolated mainly from the rumen of cattle and sheep. The aim of this study was to investigate
phenotypic characteristics that discriminate between different phylotypes. The phylogenetic position, derived from 16S rDNA
sequence data, of 45 isolates from different species and different countries was compared with their fermentation products,
mechanism of butyrate formation, lipid metabolism and sensitivity to growth inhibition by linoleic acid (LA). Three clear
sub-groups were evident, both phylogenetically and metabolically. Group VA1 typified most Butyrivibrio and Pseudobutyrivibrio isolates, while Groups VA2 and SA comprised Butyrivibrio hungatei and Clostridium proteoclasticum, respectively. All produced butyrate but strains of group VA1 had a butyrate kinase activity <40 U (mg protein)−1, while strains in groups VA2 and SA all exhibited activities >600 U (mg protein)−1. The butyrate kinase gene was present in all VA2 and SA bacteria tested but not in strains of group VA1, all of which were
positive for the butyryl-CoA CoA-transferase gene. None of the bacteria tested possessed both genes. Lipase activity, measured
by tributyrin hydrolysis, was high in group VA2 and SA strains and low in Group VA1 strains. Only the SA group formed stearic
acid from LA. Linoleate isomerase activity, on the other hand, did not correspond with phylogenetic position. Group VA1 bacteria
all grew in the presence of 200 μg LA ml−1, while members of Groups VA2 and SA were inhibited by lower concentrations, some as low as 5 μg ml−1. This information provides strong links between phenotypic and phylogenetic properties of this group of clostridial cluster
XIVa Gram-positive bacteria. |
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Keywords: | Biohydrogenation Linoleic acid Rumen |
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