Hepassocin regulates cell proliferation of the human hepatic cells L02 and hepatocarcinoma cells through different mechanisms |
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Authors: | Cao Meng-Meng Xu Wang-Xiang Li Chang-Yan Cao Chuan-Zeng Wang Zhi-Dong Yao Jia-Wei Yu Miao Zhan Yi-Qun Wang Xiao-Hui Tang Liu-Jun Chen Hui Li Wei Ge Chang-Hui Yang Xiao-Ming |
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Institution: | School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China. |
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Abstract: | Hepassocin (HPS) is a specific mitogenic active factor for hepatocytes, and inhibits growth by overexpression in hepatocellular carcinoma (HCC) cells. However, the mechanism of HPS regulation on growth of liver-derived cells still remains largely unknown. In this study, we found that HPS was expressed and secreted into the extracellular medium in cultured L02 human hepatic cells; conditional medium of L02 cells promoted proliferation of L02 cells and this activity could be blocked by anti-HPS antibody. Moreover, we identified the presence of receptor for HPS on L02 cells and HepG2 human hepatoma cells. Overproduction of truncated HPS, which signal peptide was deleted, significantly inhibited the proliferation of HCC cells and induced cell cycle arrest. These findings suggest that HPS promotes hepatic cell line L02 cells proliferation via an autocrine mechanism and inhibits HCC cells proliferation by an intracrine pathway. |
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Keywords: | HEPASSOCIN AUTOCRINE HCC INTRACRINE |
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