LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae. |
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| Authors: | T Fujii D Naka N Toyoda and H Seto |
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| Institution: | Department of Applied Biological Science, Science University of Tokyo, Chiba, Japan. |
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| Abstract: | When cells competent for genetic transformation of Streptococcus pneumoniae which could bind and enable entry of extracellular DNA molecules were treated with LiCl, they released a nickase that introduced nicks into a double-stranded DNA in the presence of EDTA. The nickase was specific for competent cells and coupled with DNA-binding activity. Furthermore, when noncompetent cells were treated with LiCl, they released the putative receptors for the competence activator. |
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