Relationships between cell membrane stability, exudate content and infectivity of Bradyrhizobium japonicum strain 639 to boron starved soybean plants |
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Authors: | Grigor Zehirov Georgi Georgiev |
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Institution: | (1) Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria |
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Abstract: | The influence of boron starvation on the root exudates content in soybean seedlings (Glycine max. L. Merr.) and the effect of exudates pretreatment on the pre-infection processes in symbiotic system Br. japonicum strain 636 and soybean were investigated. Root cell membrane stability of boron starved soybean plants (-B) decreased compared
to the control. The concentrations of all analyzed metabolites (reducing sugars, free amino acids, organic acids, soluble
phenols and total flavonoids) from root exudates of -B plants were lower than the control concentrations. Analysis of polyphenols
after HPLC chromatography of root exudates showed significant difference of peak numbers between chromatograms of exudates
obtained from boron starved and from control plants.
Bacterial culture treatment with root exudates from -B plants showed decreased growth, chemotaxis and attachment ability toward
the host root compared to the control exudate treatments. These changes were accompanied by decreased nodulation and acetylene
reduction activity of boron starved soybean plants. |
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Keywords: | attachment ability boron starvation Bradyrhizobium chemotaxis N2-fixation root exudates soybean |
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