New exopolysaccharides produced by Aureobasidium pullulans grown on glucosamine |
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| Authors: | Cescutti Paola Pupulin Raffaella Delben Franco Abbate Maria Dentini Mariella Sparapano Lorenzo Rizzo Roberto Crescenzi Vittorio |
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| Affiliation: | Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università di Trieste, via L. Giorgieri 1, I-34127, Trieste, Italy. |
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| Abstract: | The polysaccharides produced by Aureobasidium pullulans, grown using glucosamine as the carbon source, were investigated by means of methylation analysis, affinity chromatography and NMR spectroscopy. The results indicated that, besides a small amount of pullulan, this micro-organism was capable of producing-in low yields-mixtures of at least two different complex polysaccharides containing mainly mannose and galactose. (1)H NMR spectra of two fractions obtained by lectin affinity chromatography indicated that one polymer was constituted exclusively of mannose residues while the other contained both galactofuranosyl and mannopyranosyl residues. |
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| Keywords: | Aureobasidium pullulans Polysaccharides Structure NMR spectroscopy Lectin affinity chromatography |
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