Genotype effects on proliferative embryogenesis and plant regeneration of soybean |
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Authors: | M A Bailey H R Boerma W A Parrott |
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Institution: | (1) Department of Crop and Soil Sciences, The University of Georgia, 30602 Athens, Georgia |
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Abstract: | Summary Proliferative somatic embryogenesis is a regeneration system suitable for mass propagation and genetic transformation of soybean
Glycine max (L.) Merr.]. The objective of this study was to examine genotypic effects on induction and maintenance of proliferative embryogenic
cultures, and on yield, germination, and conversion of mature somatic embryos. Somatic embryos were induced from eight genotypes
by explanting 100 immature cotyledons per genotype on induction medium. Differences in frequency of induction were observed
among genotypes. However, this step was not limiting for plant regeneration because induction frequency in the least responding
genotype was sufficient to initiate and maintain proliferative embryogenic cultures. Six genotypes selected for further study
were used to initiate embryogenic cultures in liquid medium. Cultures were evaluated for propagation of globular-stage tissue
in liquid medium, yield of cotyledon-stage somatic embryos on differentiation medium, and plant recovery of cotyledon-stage
embryos. Genotypes also differed for weight and volume increase of embryogenic tissue in liquid cultures, for yield of cotyledon-stage
embryos on differentiation medium, and for plant recovery from cotyledon-stage embryos. Rigorous selection for a proliferative
culture phenotype consisting of nodular, compact, green spheres increased embryo yield over that of unselected cultures, but
did not affect the relative ranking of genotypes. In summary, the genotypes used in this study differed at each stage of plant
regeneration from proliferative embryogenic cultures, but genotypic effects were partially overcome by protocol modifications. |
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Keywords: | somatic embryogenesis conversion desiccation Glycine max |
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