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Enhanced activity of Mucor javanicus lipase in polyoxyethylene sorbitan trioleate containing microemulsion-based organogels |
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| Authors: | Kazuhito Nagayama Masanao Imai |
| Institution: | aDepartment of Materials Science and Engineering, Kochi National College of Technology, 200-1 Monobe, Nankoku, Kochi 783-8508, Japan bDepartment of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan |
| Abstract: | The efficacy of polyoxyethylene sorbitan trioleate (Tween 85) addition on the activity of Mucor javanicus lipase was investigated in sodium bis(2-ethylhexyl) sulfosuccinate (AOT) microemulsion-based organogels (MBGs). Gelatin was used as the gelling component of the MBGs. The maximal reaction rate was obtained at an AOT:Tween 85 molar ratio of 10:1. Under a fixed molar ratio system of AOT:Tween 85 = 10:1, the reaction rate also attained a maximum at a WG (=H2O]/AOT + Tween 85] in MBG phase) value of 100 and an AOT concentration of 150 mM. The reaction proceeded under a reaction-controlled regime, and the reaction rate for the AOT/Tween 85 mixed system was about 2-fold higher than that for the AOT single system. The lipase activity was well maintained for 10 days and recovered by contacting the MBGs with concentrated amphiphile solutions. |
| Keywords: | AOT Tween 85 Microemulsion Gelatin Organogels Lipase |
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