在昆虫细胞中同时表达蓝舌病毒VP3与VP7蛋白可装配成核心样颗粒

将蓝舌病毒（ＢＴＶ）１３型Ｓ７与Ｌ３基因同时插入杆状病毒双表达载体ｐＥａｓｔＢａｃＤｕａｌ，获得重组杆状病毒ｒｖＢａｃＢＴＶＰ３７。该病毒在昆虫细胞中同时高水平表达ＢＴＶ１３ ＶＰ３与ＶＰ７蛋白，可以高效自动装配出２０面体的６０￣７０ｎｍ空心颗粒。分析表明，所获颗粒为空心的ＢＴＶ核心样颗粒（ＣＬＰ），其成分为ＶＰ３与ＶＰ７，不含ＢＴＶ其它任何蛋白与核酸。这种装配需要ＶＰ３与ＶＰ７的共同参与，二者缺

Assembly of Core-like Particles of Bluetongue Virus by co-expressing the lnner Core Proteins VP3 and VP7 in Inset Cells

Bluetongue virus (BTV) 13 VP3 and VP7 proteins were co-expressed in Sf-9 cells at a high level through inserting them into a dual baculovirus expression vector pFastBacDual. The results showed that many empty 60-70nm core-like particles could be found after purification of the extracts by ultracentrifugation from dual recombinant baculovirus infected cells. Negative staining showed that the expressed particles were similar to authentic BTV cores but different from intact BTV virions. Further analysis showed that neither any RNA/DNA species nor other BTV proteins except VP3 and VP7 could be detected in the particles. The particles could be visualized in both cytoplasm and nuclei of Sf-9 cells. Our results implied that VP3 and VP7 are essential for the assembly of core-like particles, the two proteins contain all the informations for assembly. This work will be the basis for assembly of BTV virus-like particles and also provides a good model for elucidating the relationship between structure and function of BTV.