Silver-Zinc Redox-Coupled Electroceutical Wound Dressing Disrupts Bacterial Biofilm |
| |
| Authors: | Jaideep Banerjee Piya Das Ghatak Sashwati Roy Savita Khanna Craig Hemann Binbin Deng Amitava Das Jay L Zweier Daniel Wozniak Chandan K Sen |
| |
| Institution: | Comprehensive Wound Center, Davis Heart & Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of America.; University of North Dakota, UNITED STATES, |
| |
| Abstract: | Pseudomonas aeruginosa biofilm is commonly associated with chronic wound infection. A FDA approved wireless electroceutical dressing (WED), which in the presence of conductive wound exudate gets activated to generate electric field (0.3–0.9V), was investigated for its anti-biofilm properties. Growth of pathogenic P. aeruginosa strain PAO1 in LB media was markedly arrested in the presence of the WED. Scanning electron microscopy demonstrated that WED markedly disrupted biofilm integrity in a setting where silver dressing was ineffective. Biofilm thickness and number of live bacterial cells were decreased in the presence of WED. Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED. This work provides first electron paramagnetic resonance spectroscopy evidence demonstrating that WED serves as a spontaneous source of reactive oxygen species. Redox-sensitive multidrug efflux systems mexAB and mexEF were repressed by WED. Taken together, these observations provide first evidence supporting the anti-biofilm properties of WED. |
| |
| Keywords: | |
|
|
