MiR-20a Promotes Cervical Cancer Proliferation and Metastasis In Vitro and In Vivo |
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| Authors: | Shan Zhao Desheng Yao Junying Chen Nan Ding Fei Ren |
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| Affiliation: | 1. Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.; 2. Department of Gynecology, Qianfoshan Hospital Affiliated to Shandong University, Jinan, China.; The University of Hong Kong, CHINA, |
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| Abstract: | MicroRNAs (miRNAs) are small, non-coding RNAs that are critical regulators of various diseases. MicroRNA-20a (miR-20a) has previously significantly altered in a range of cancers. In this study, we detected the relationship between miR-20a and the development of cervical cancer by qRT-PCR, we found that the expression level of miR-20a was significantly higher in cervical cancer patients than in normal controls, the aberrant expression of miR-20a was correlated with lymph node metastasis, histological grade and tumor diameter. Then we successfully established the stable anti-miR-20a cervical cancer cell lines by lentivirus. Inhibited miR-20a prevented tumor progression by modulating cell cycle, apoptosis, and metastasis in vitro and in vivo. TIMP2 and ATG7 were proved to be direct targets of miR-20a, using luciferase assay and western blot. These results indicate that miR-20a suppresses the proliferation, migration and invasion of cervical cancer cell through targeting ATG7 and TIMP2. Our results support the involvement of miR-20a in cervical tumorigenesis, especially lymph node metastasis. We propose that miRNAs might be used as therapeutic agent for cervical cancer. |
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