四种细菌L型的形态定量分析

用抗生素或臭氧的方法诱导出四种细菌和Ｌ型后，用图像分析技术定量检测Ｌ型多形性，表面积、最大直径、周长、等效圆直径和形状因子，并作比较分析。四种细菌Ｌ型及Ｌ型中的巨形体在上述５项形态学参数指标上除形状因子外均较细菌型明显增大，而形状因子参数明显小于细菌型。细菌Ｌ型之间在形态学指标上的差异有显著性（ｐ＜０．０５）。形态定量分析能准确客观地反映形态定性观察的结果以及细菌Ｌ型的形态变异程度，为细菌Ｌ型的形态定量分析提供依据。

MORPHOLOGICAL QUANTITATIVE ANALYSIS OF BACTERIAL L-FORME

After four species of bacteria were induced into L-form by the meanes of antibiotics orozone,the image analysis technique was used to analysed their area,d_(max),d_(circle),perim.and f_(shape),aswell as tremendous bodies of the L-forms.The abov-mentioned 5 terms of morphological parame-ters had increase significantly with a variety of 0.29-13 m more than those of the bacterial typesonly except the f_(shape) parameters.The f_(shape) paramters of the L-forms were evidently smaller thanthose of bacterial types.The morphological parameters among bacterial L-forms had a marked dif-ference(p 0.05).So this suggested that morphological quantitative analysis could accurately andobjecti-vely reflect the results of morphological qualitative observation and morphological varia-tion degrees of bacterial L-forms.It also could provide a basis for morphological quantitative anal-ysis of bacterial L-forms.