Identification of microspore-derived plants in anther culture of flax (Linum usitatissimum L.) using molecular markers |
| |
| Authors: | Y Chen G Hausner E Kenaschuk D Procunier P Dribnenki G Penner |
| |
| Institution: | (1) Agriculture and Agri-Food Canada, Morden Research Centre, Unit 100-101 Route 100, Morden, Manitoba, R6M 1Y5, Canada, CA;(2) Agriculture and Agri-Food Canada, Cereal Research Centre, 195 Dafoe Road, Winnipeg, Manitoba, R3T 2M9, Canada, CA;(3) United Grain Growers Limited, Linola Breeding Program, Unit 100-101, Morden, Manitoba, R6M 1Y5, Canada, CA |
| |
| Abstract: | The microspore origin of anther-culture-derived plants of flax was determined using inter-simple sequence repeat (ISSR) and
randomly amplified polymorphic DNA (RAPD) markers. Polymorphic fragments between the two parents of the F1 donor plants were identified and their segregation patterns in anther-culture-derived plants were used to elucidate the origin
of those plants and to determine the degree of independence of plants regenerated from the same callus. Using one ISSR primer
(UBC 889) and two RAPD primers (UBC 556 and 561), 12 out of 16 plants were unequivocally identified as being derived from
microspores. Plants derived from the same callus had identical PCR patterns at five polymorphic loci and thus were likely
derived from the same microspore. Therefore, it is proposed that the number of calli forming shoots be used to describe the
anther culture efficiency in flax.
Received: 3 February 1998 / Revision received: 8 June 1998 / Accepted: 8 July 1998 |
| |
| Keywords: | Anther culture Flax ISSRs Linum usitatissimum RAPDs |
| 本文献已被 SpringerLink 等数据库收录！ |
|
