The complete amino acid sequence of the murine transplantation antigen H-2Db as deduced by molecular cloning |
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Authors: | Antonio A Reyes Monica Schöld R Bruce Wallace |
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Institution: | (1) Department of Molecular Genetics, City of Hope Research Institute, 91010 Duarte, California;(2) Present address: Division of Biology, California Institute of Technology, 91125 Pasadena, California |
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Abstract: | A mouse cDNA library derived from the EL4 cell line (b-haplotype) was screened with a probe containing a small part of the H-2Kb coding region. One of the clones isolated, pH203, encodes a protein whose deduced amino acid sequence is identical with the known sequence of H-2Db in 141 of 141 positions available for comparison. The clone, therefore, is believed to code for the H-2Db transplantation antigen. The cDNA insert of pH203 contains the coding region for residues 82 through the carboxy-terminus of H-2Db, and includes 476 nucleotides of the 3-untranslated sequence. Comparison between the H-2Db cDNA clone and a previously isolated H-2Kb cDNA clone shows homologies of 83% and 91% at the amino acid and nucleotide levels, respectively. Analysis of DNA sequences at the 3-coding and untranslated regions suggests that the mRNAs of H-2Kb and H-2Db are spliced differently at their 3-coding ends. |
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