Genetic structure of a morphological species within the amoeba genus Korotnevella (Amoebozoa: Discosea), revealed by the analysis of two genes |
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| Affiliation: | 1. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaja nab. 7/9, 199034, St. Petersburg, Russia;2. Department of Genetics and Evolution, University of Geneva, Sciences III, 1211 Geneva, Switzerland;1. Limnological Station, Institute of Plant Biology, University of Zurich, Seestrasse 187, CH-8802 Kilchberg, Switzerland;2. Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Hydrobiology, Na Sádkách 7, CZ-37005 České Budějovice, Czech Republic;3. Department of Zoology, Institute for Biomaterials and biomolecular Systems, University of Stuttgart, Pfaffenwaldring 57, D-70569 Stuttgart, Germany;1. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaja nab. 7/9, 199034, St. Petersburg, Russia;2. Department of Organismal Biology, Systematic Biology Program, Uppsala University, Kåbovägen 4A, SE-752 36, Uppsala, Sweden;1. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaja nab. 7/9, 199034, St. Petersburg, Russia;2. Department of Organismal Biology, Systematic Biology Program, Uppsala University, Kåbovägen 4A, SE-752 36, Uppsala, Sweden;1. Laboratory of Cellular and Molecular Protistology, Zoological Institute RAS, 199034 St. Petersburg, Russia;2. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, 199034 St. Petersburg, Russia;3. “Persistence of Microorganisms” Center of Shared Scientific Equipment, Institute for Cellular and Intracellular Symbiosis UrB RAS, 460000 Orenburg, Russia;1. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, Universitetskaja nab. 7/9, 199034 St. Petersburg, Russia;2. Laboratory of Cellular and Molecular Protistology, Zoological Institute RAS, Universitetskaja nab. 1, 199034 St. Petersburg, Russia;3. Interdisciplinary Resource Center for Nanotechnology, Research Park, St. Petersburg State University, Universitetskaja nab. 7/9, 199034 St. Petersburg, Russia |
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| Abstract: | Amoebae of the genus Korotnevella are covered with scales, the structure of which is believed to be species–specific and allows distinguishing species reliably at the morphological level. We studied members of this genus in order to assess the genetic structure of the local populations of amoebae. For the present study we isolated nine freshwater strains of Korotnevella, belonging to three species, from two locations in North-Western Russia. In order to obtain data on the population structure of these amoebae, we identified all isolates based on the light-microscopic morphology and scale structure and investigated both inter-strain and intra-strain polymorphism of Cox I and 18S rRNA genes. Results show that both genes provide congruent patterns of population structure. The Cox I gene appears to be more reliable DNA barcode while the 18S rRNA gene shows an interesting pattern of polymorphism, which may represent phylotypes of amoebae. Local population of amoebae in every studied species consists of a number of genetic lineages (phylotypes), some shared between the populations while others are unique to a local habitat. |
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| Keywords: | Amoebae Cox I Phylogeny Small-subunit rRNA Species structure |
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