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Hematopoiesis-dependent expression of CD44 in murine hepatic progenitor cells |
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| Authors: | Shinya Ohata Makiko Nawa Tokiwa Yamasaki Kenji Sawanobori Shoji Hata Yoichi Asaoka Hitoshi Okamoto Shuji Terai Toshiaki Katada |
| Affiliation: | a Department of Developmental and Regenerative Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan b Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan c Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute, Saitama, Japan d Laboratory of Cytometry and Proteome Research, Tokyo Medical and Dental University, Tokyo, Japan e Graduate School of Humanities and Sciences School of Natural Science and Ecological Awareness, Nara Women’s University, Kitauoyahigashi-machi, Nara, Japan f Department of Tumor Biochemistry, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan g Department of Gastroenterology and Hepatology, School of Medicine, Yamaguchi University, Yamaguchi, Japan |
| Abstract: | The fetal liver serves as the predominant hematopoietic organ until birth. However, the mechanisms underlying this link between hematopoiesis and hepatogenesis are unclear. Previously, we reported the isolation of a monoclonal antibody (anti-Liv8) that specifically recognizes an antigen (Liv8) present in murine fetal livers at embryonic day 11.5 (E11.5). Liv8 is a cell surface molecule expressed by hematopoietic cells in both fetal liver and adult mouse bone marrow. Here, we report that Liv8 is also transiently expressed by hepatoblasts at E11.5. Using protein purification and mass spectrometry, we have identified Liv8 as the CD44 protein. Interestingly, the expression of Liv8/CD44 in fetal liver was completely lost in AML1−/− murine embryos, which lack definitive hematopoiesis. These results show that hepatoblasts change from Liv8/CD44-negative to Liv8/CD44-positive status in a hematopoiesis-dependent manner by E11.5, and indicate that Liv8/CD44 expression is an important link between hematopoiesis and hepatogenesis during fetal liver development. |
| Keywords: | Liver Hepatogenesis Hepatoblast Hematopoiesis Monoclonal antibody Liv8 CD44 AML1 AGM Erythrocyte Liver development |
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