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Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade |
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| Authors: | Ikuo Kimura Morichika Konishi Naoko Furukawa Masahiro Mori Gozoh Tsujimoto Nobuyuki Itoh |
| Institution: | a Laboratory of Applied Pharmacology, Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba 288-0025, Japan b Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Science, Sakyo, Kyoto 606-8501, Japan c Department of Genomic Drug Discovery Science, Kyoto University Graduate School of Pharmaceutical Science, Sakyo, Kyoto 606-8501, Japan d Department of Medical Biochemistry, Kobe Pharmaceutical University, Higashinada, Kobe 658-8558, Japan |
| Abstract: | Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed adipogenesis in 3T3-L1 cells. The knockdown of neudesin by RNA interference markedly promoted adipogenesis in 3T3-L1 cells and decreased MAPK activation during adipocyte differentiation. The addition or knockdown of neudesin affected the expression of C/EBPα and PPARγ but not of C/EBPβ. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBPβ induces PPARγ and C/EBPα expressions, by controlling the MAPK pathway. |
| Keywords: | Adipocytes Adipogenesis 3T3-L1 Neudesin Heme-binding protein Obesity Heme MAPK |
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