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Positive modulation of RNA polymerase III transcription by ribosomal proteins |
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| Authors: | Giorgio Dieci Roberta Ruotolo Christophe Carles Angela Amoresano |
| Affiliation: | a Dipartimento di Biochimica e Biologia Molecolare, Università degli Studi di Parma, Viale G.P. Usberti 23/A, 43100 Parma, Italy b Commissariat à l’Énergie Atomique, Institut de Biologie et de Technologies de Saclay, SBIGeM, F-91191 Gif sur Yvette Cedex, France c Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Via Cinthia 4, 80126 Napoli, Italy |
| Abstract: | A yeast nuclear fraction of unknown composition, named TFIIIE, was reported previously to enhance transcription of tRNA and 5S rRNA genes in vitro. We show that TFIIIE activity co-purifies with a specific subset of ribosomal proteins (RPs) which, as revealed by chromatin immunoprecipitation analysis, generally interact with tRNA and 5S rRNA genes, but not with a Pol II-specific promoter. Only Rpl6Ap and Rpl6Bp, among the tested RPs, were found associated to a TATA-containing tRNAIle(TAT) gene. The RPL6A gene also emerged as a strong multicopy suppressor of a conditional mutation in the basal transcription factor TFIIIC, while RPL26A and RPL14A behaved as weak suppressors. The data delineate a novel extra-ribosomal role for one or a few RPs which, by influencing 5S rRNA and tRNA synthesis, could play a key role in the coordinate regulation of the different sub-pathways required for ribosome biogenesis and functionality. |
| Keywords: | RNA polymerase III tRNA Ribosomal proteins Saccharomyces cerevisiae TFIIIE |
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