Characterization of gingival epithelium epidermal growth factor receptor |
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| Authors: | S Sengupta J Liu S L Wang E Piotrowski A Slomiany B L Slomiany |
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| Institution: | Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400. |
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| Abstract: | The binding characteristics of gingival epithelium epidermal growth factor (EGF) receptor were investigated using epithelial cell membranes from bovine gingiva. The binding of 125I]EGF was found to be time and protein concentration dependent, reversible, and specific. Unlabeled EGF competed for 125I]EGF binding with IC50 of 0.25nM and maximum displacement of 93% at 0.81nM. Scatchard analysis of the binding data inferred the presence of two binding sites, one of high affinity (Kd = 3.3 nM and Bmax = 47.3fmol/mg protein) and the other of a low affinity (Kd = 1.6 microM and Bmax = 1.9pmol/mg protein). Crosslinking of 125I]EGF to gingival membranes followed by polyacrylamide gel electrophoresis and autoradiography revealed a receptor protein of 170kDa. |
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