胰高血糖素样肽-2对小鼠小肠缺血/再灌注损伤的保护作用

目的:观察胰高血糖素样肽-2(GLP-2)对缺血/再灌注损伤小鼠小肠的保护效应.方法:采用肠缺血/再灌注(I/R)模型,将32只小鼠随机分为4组(n=8)假手术(Sham)组、I/R组、I/R GLP-2保护组和I/R 谷氨酰胺(GLN)阳性对照组.光镜观察小肠黏膜形态学改变.检测小肠绒毛高度和隐窝深度;小肠组织二胺氧化酶(DAO)活性;肠系膜淋巴结(MLN)细菌易位率.结果:与假手术组相比,I/R组部分小肠绒毛坏死脱落,绒毛高度下降,隐窝变浅(P＜0 01);小肠组织DAO活性降低(P＜0.01);MLN细菌易位率增加(P＜0.05).与I/R组比,GLP-2组肠绒毛损害明显减轻,DAO活性回升(P＜0.01),细菌易位率回降(P＜0.05).结论:GLP-2对缺血/再灌注损伤小鼠小肠的形态结构及肠屏障功能具有保护作用.

Protective effects of glucagon-like peptide 2 on intestinal ischemia/reperfusion injury in mice

Aim: To investigate the protective effects of glucagon-like peptide 2(GLP-2) on intestinal ischemia/reperfusion(I/R) injury in mice. Methods: Intestinal ischemia/reperfusion model in mice were set up and 32 mice of Kunming species were divided randomly into 4 groups(n=8): Sham group, I/R group, I/R GLP-2 group and I/R glutamine group. The morphologic changes of intestinal mucosa were observed under LM. The villus height and crypt depth of intestine, the activity of diamine oxidase(DAO) in intestine and bacterial translocation rates of mesenteric lymph nodes(MLN) were detected. Results: Compared with sham operation group, the intestinal villi were sloughed in I/R group with decreased villus height and crypt depth(P<0.01), the DAO activities were decreased(P<0.01), and MLN bacterial translocation rates were increased(P<0.05). While GLP-2 administration improved the villus damage, increased DAO activity(P<0.01), and decreased MLN bacterial translocation rates(P<0.05), compared with I/R group. Conclusion: GLP-2 have protective effects on intestinal morphology and barrier function after ischemia/reperfusion injury in mice.