Induction,growth and direct rooting of adventitious shoots of Begonia x hiemalis |
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Authors: | J Simmonds |
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Institution: | (1) Ottawa Research Station, Agriculture Canada, K1A 0C6 Ottawa, Ontario, Canada |
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Abstract: | The efficiency of commercial micropropagation programs for Begonia x hiemalis depends on the production of large adventitious shoots for easy handling and on effective rooting and acclimatization procedures. Maximum induction of adventitious buds on petiole segments occurred in response to NAA (0.1 mg, l-1) and BA (0.5 mg l-1), but continued shoot growth was limited. With a lower concentration of BA (0.1 mg l-1) fewer shoots were produced but shoot growth was enhanced. With a combined agar/liquid culture program the low BA (0.1 mg l-1) medium produced 50 percent more shoots larger than 1 cm than did the high BA (0.5 mg l-1) medium. In vitro rooted explants developed weak root systems and acclimatization losses occurred during adaptation to greenhouse conditions. Adventitious shoots treated with commercial rooting powder and placed directly in mist frames produced much stronger root systems and could be adapted to greenhouse conditions without loss. The elimination of the in vitro rooting stage also simplifies the micropropagation program.Contribution No. 743 |
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Keywords: | Tissue culture micropropagation organogenesis |
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